Proposal-submission-to-beamline

Beamline 1.3W · Small and Wide Angle X-ray Scattering (SAXS/WAXS)

Proposal Submission to Beamline

Estimate Your Beamline Shifts

Estimating beamtime accurately ensures efficient scheduling and resource allocation at Beamline 1.3W. Users can evaluate their required shifts based on the guidelines below.

1. Setup & Instrument Time

This includes the time needed before starting actual sample measurements.

Beamline Setup (including training)

SAXS only: 3 hours
WAXS only: 1.5 hours
SWAXS (simultaneous): 4 hours

Instrument Setup (accessories)

Cooler: 30 minutes
Temperature control: 30 minutes
Tensile: 1 hour
Humidity: Please contact Dr. Siriwat for time estimation.

2. Sample Measurement Time

Exposure time varies depending on the sample state.

Solid Samples

Exposure time: 0.5 – 10 minutes / sample
Background time: 0.5 – 10 minutes (Shared background can be used in some cases)

Liquid Samples

Exposure time: 10 minutes / sample
Background time: 10 minutes / solvent type

Special Requirements for Protein Solutions

Proteins with MW < 50 KDa: Concentration must be > 2 mg/mL (recommended 4-10 mg/mL)
Proteins with MW > 50 KDa: Concentration 2-10 mg/mL is adequate
Important: Each protein sample must be measured at least 3 concentrations.

Heating Condition Experiments: Please spare time for cooling down the sample stage before mounting the next sample.

3. Example Calculations

Typically, the total calculated time is rounded to estimate the number of shifts.

Example 1: General Powder Samples

Condition: 10 Starch samples, no temperature control, objective: Lamellar and Crystallinity (SAXS & WAXS)

Items	Time (hrs)
SAXS Setup	3
Powder (SAXS)	10*10 min = 1.5 hrs + background 0.5 hrs
WAXS Setup	1.5
Powder (WAXS)	10*10 min = 1.5 hrs + background 0.5 hrs
Total Time	3 + 2 + 1.5 + 2 = 8.5 hrs ≈ 1 Shift

Example 2: Protein Solution

Condition: 1 Protein sample (100 uL, requires 3 concentrations), measuring SAXS.

Items	Time (hrs)
SAXS Setup	3
Protein Solution	3*10 min = 0.5 hrs + background 0.5 hrs
Sample Prep.	3*(10(centrifuge) + 10(walking)) = 1 hr
Total Time	3 + 1 + 1 = 5 hrs ≈ 1 Shift

Example 3: In-situ Experiment (Tensile testing)

Condition: 10 Rubber samples, room temperature, Tensile speed: 10 mm/min (max extend 300 mm from 400 mm limit), objective: SIC/orientation (WAXS + Tensile)

Items	Time (hrs)
WAXS + Tensile Setup	1.5 + 1
In-situ Experiment	1030 min(extend) + 101 min(expose) = 5 hrs 10 min + background 10*1 min
Sample Prep.	10*10 = 100 min
Total Time	2.5 + 5.33 + 1.66 = 9.5 hrs ≈ 1 Shift