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Description

Infrared microspectroscopy is widely used for chemical compound identification. Thanks to the high brightness of synchrotron radiation, synchrotron based Infrared microspectroscopy provides high spatial resolution, better signal to noise ratio and shorter data acquisition time than the conventional source.

BL 4.1 IR spectroscopy and Imaging is designed to extract the far- to mid- infrared light (spectral range between 4000-100 cm-1) from the 1.2 GeV Siam Photon Source. This beamline can be divided into 3 branches in order to provide 3 end-stations working simultaneously. 

Technical specifications
Photon Energy range Between 0.01-0.5 eV
Wavelength 2.5-100 microns
Source Edge and Bending Magnet Radiation 
Objective

36X Schwarzschild Objective

20X ATR Objective

Spectrometer/microscope  Vertex 70 spectrometer/Hyperion 2000 microscope
Polarization  Both linearly (bending magnet radiation) and radially polarized (edge radiation)
Beam size at sample  10X10 µm2
Detector  100 micron Narrow band MCT
Mode of measurement

Reflection,

Transmission

Attenuated Total Reflectance (ATR)

       

Signal-to-Noise Comparison

Infrared spectra on sample collected with 10x10 µm square aperture, the brightness advantage of the synchrotron has significantly improved the signal-to-noise ratio.

 

 

 

 

 

 

 

 

 

 

 

 

 

  Equipments

 

Objectives:

  • 36X Schwarzschild Objective

 

Available technique Research field
36X objective of Transmission

Biomedical science

Food and Agricultural science

Polymer science

36X objective of Reflection

Biomedical science

Food and Agricultural science

Polymer science

36X objective of Transmission & Diamond avil cells

Contamination

Material science

36X objective of Transmission & Vis polarized

Biomedical science

Material science

ATR 20X

Biomedical science

Environmental science

Polymer science

 

 

Sample preparation and requirement

 

SR-FTIR Sample Techniques - transmission

Excellent quality spectra can be obtained for many types of samples using transmission mode of experiment. The transmission technique can be used combine with 36X microscopes to analyze:

What types of samples can you analyze?

Biological sample/Tissue

The transmission mode requires for the spectra acquisition and careful preparation of the samples. For biological samples, cross section is usually recommended. The sample will be embedded in paraffin or OCT (optimum cutting temperature) then microtome or cryostat into thin cross section (Tissue cross section should not more than 7 micron). The sample will be put on window and placed in a desiccator loaded with silica gel for at least 24 hours before analysis. However, paraffin has most intense absorbance features from CH stretching vibration (3000-2800 cm-1) and CH2, CH3 bending (1460 and 1380 cm-1). Therefore, it was usual to remove paraffin from the sample by washing of xylene before FTIR analysis.

Before coming, it is importantly recommended to test the samples with SR-FTIR microspectroscopy. This can enable to check the sample is thickness enough or user can prepare of different thickness for testing.

Bacterial cell

Cell suspensions in media will be washed with normal saline (0.85%) to remove media then will be washed in sterile distilled water 2 times and then deposit into BaF2 window (1-2 µl/drop on window). The samples will be make a smooth layer of the sample in desiccated under vacuum for several hours and stored in desiccators to form films suitable before analysis.

 

Using Remote Connection via VPN

 

Using VPN, you can use software (Cytospec, OPUS and The UnscramblerX) to do the data analysis and control a computer from a different computer network.

 

  • VPN access for user have been approved by the Beamline manager. Completed application forms must be submitted one weeks prior to being granted remote access on network. Please send the complete form to This email address is being protected from spambots. You need JavaScript enabled to view it.
  • User can check your booking time via this link: http://www.slri.or.th/th/index.php/beamline/bl41.html?view=article&id=6014:booking-calendar-for-using-data-analysis-software-via-vpn&catid=56:bl41-ir

 

Beam time on this beamline/ How to apply for beam time

 

If user interested in applying for beam time first step is contact the beamline scientist to discuss the experiment or user office group leader. The next step is to submit a proposal, register as new user or sign in using your username and password. The application for apply the beam time should be including main information following details:

Ø  How the SR-FTIR experiment fit into your overall project.
Ø  Explain the proposed experimental work giving information that will allow to establish the feasibility and outcomes.
Ø  The detailed of sample information and preparation.
Ø  The experimental design or  spectrum acquisition parameters (For example: Spectral range, Number of scans and resolution)
Ø  List the people who will participate in the experiments (1 shift = 8 hours, 3 shifts/day) Service

User will get a small amount of beam time to test the feasibility of new samples.

Proposal submission

Click: http://beamapp.slri.or.th/

   

BL 4.1 IR staff

 

Beamline manager 

 

Dr. Kanjana Thumanu,  Email : This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. 044-217040 ext.1487

Beamline scientist  

Dr. Buabarn Kuaprasert,  Email : This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. 044-217040 ext.1489    
Dr. Supatcharee Siriwong, Email : This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. 044-217040 ext. 1660 
Dr. Kanokwan Kamkajon, Email : This email address is being protected from spambots. You need JavaScript enabled to view it.,  Tel. 044-217040 ext. 1660

Assistant beamline researcher 

Duangjai Srisamut, Email : This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. 044-217040 ext. 1661  
Chulalak Chantarakon, Email : This email address is being protected from spambots. You need JavaScript enabled to view it.,  Tel. 044-217040 ext. 1660

 

 

 

Should you have any technical questions, please do not hesitate to contact us via e-mail or tel: ++66 44 217040 ext 1660-1661

For further information about proposal submission, please contact SLRI User Office, useroffice  or  ++66 44 217040 ext 1601-1605

Useful link for infrared spectroscopy technique

Infrared beamlines around the world

Beamline around the world

Synchrotron facility Country Website
Canadian Light Source (CLS) Canada

http://midir.lightsource.ca/

http://www.lightsource.ca/experimental/farir.php

Advanced Light Source (ALS) USA http://www-als.lbl.gov/index.php/beamlines/beamlines-directory.html
European Synchrotron Radiation Facility (ESRF) France http://www.esrf.eu/UsersAndScience/Experiments/Imaging/ID21/SrFtir/FtirIntro
Australian Synchrotron Australia http://www.synchrotron.org.au/index.php/aussyncbeamlines/infrared-micro/introduction
Diamond Light Source UK http://www.diamond.ac.uk/Home/Beamlines/B22.html
Synchrotron Radiation Center (SRC) USA http://www.src.wisc.edu/news/stories/IRENI.08.13.08.htm
ELETTRA Italy http://www.elettra.trieste.it/it/lightsources/elettra/elettra-beamlines/sissi/sissi.html
ANKA(Ångströmsquelle Karlsruhe) Germany http://ankaweb.fzk.de/_cms/_release/instrumentation_at_anka/beamlines.php?id=1
SPring-8 Japan http://www.spring8.or.jp/wkg/BL43IR/instrument/lang-en/INS-0000001427/instrument_summary_view
MAX-Lab Sweden https://www.maxlab.lu.se/node/45
National Synchrotron Radiation Laboratory (NSRL) China http://en.nsrl.ustc.edu.cn/Machine_Beamlines/Beamlines/201107/t20110701_114972.html
Synchrotron-SOLEIL France http://www.synchrotron-soleil.fr/Recherche/LignesLumiere/SMIS
Swiss Light Source (SLS) Switzerland http://www.psi.ch/sls/ir/ir
ALBA (synchrotron) Spain http://www.miras2.es/que.html
Synchrotron-light for Experimental Science and Applications in the Middle East (SESAME) Jordan http://www.sesame.org.jo/sesame/machine-and-beamlines/beamlines/phase-1/day-1-bl/infrared-beamline-emira.html

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